微型聚合酶反應(yīng)芯片的精確溫度控制

微型PCR 反應(yīng)芯片是生物芯片的一個(gè)重要發(fā)展方向，是實(shí)現(xiàn)快速分子生物學(xué)檢測(cè)的一個(gè)新興技術(shù)。在微型PCR 芯片中，變性、回火、延伸三個(gè)溫度區(qū)的精確控制直接影響到
生成產(chǎn)物的數(shù)量和特異性，因此至關(guān)重要。本文使用虛擬儀器技術(shù)構(gòu)建溫度測(cè)控系統(tǒng),將其應(yīng)用于微型PCR 芯片的精確溫度控制,該系統(tǒng)精度高, 溫度波動(dòng)較小。通過Labview 圖形化編程軟件和數(shù)據(jù)采集卡,大大縮短了系統(tǒng)組建時(shí)間，使溫度數(shù)據(jù)采集和顯示一次性完成。
關(guān)鍵字：聚合酶反應(yīng)； Labview； 溫度控制


Abstract: Micro PCR chip is a new development of Biochips. By this novel method, precise
detection in molecular biology field can be realized rapidly. It is very important for micro PCR
chip to accurately control the temperature of melting, annealing and extension. It will influence
both the production and the specificity of result directly. In this paper, graphical development
software and DAQ data acquisition card has been used to realize high precise and less fluctuant temperature control on chip, which not only contribute to accomplish data display and acquisition simultaneously, but shorten time for system construction as well.
Key words:PCR,temperature control,Labview